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为了解脑出血对脑细胞内Ca2 + 浓度的影响及羚蝎胶囊的干预作用 ,采用胶原酶复制大鼠脑出血模型 ,通过胰蛋白酶消化制备脑细胞悬液 ,以Fura -2 /AM为荧光指示剂 ,应用双波长荧光分光光度计测定用羚蝎胶囊 (羚羊角、全蝎、三七、水蛭等 )前后大鼠脑细胞内Ca2 + 浓度。结果 :在细胞外Ca2 + 浓度为 1mmol/L情况下 ,正常组大鼠脑细胞内Ca2 + 浓度为 32 0± 83nmol/L ,假手术组为 35 5± 48nmol/L ,模型组为 10 10± 118nmol/L ,羚蝎胶囊组为 930± 84nmol/L ,清开灵组为 910± 5 9nmol/L。提示脑出血大鼠脑细胞内存在严重的Ca2 + 超载现象 ,羚蝎胶囊能有效地降低脑出血大鼠脑细胞内Ca2 +浓度
In order to understand the effect of intracerebral hemorrhage on intracellular Ca2+ concentration in brain cells and the intervention of antelope capsule, collagenase was used to replicate the model of intracerebral hemorrhage in rats, and brain cell suspension was prepared by trypsin digestion. The fluorescence intensity was indicated by Fura-2/AM. A two-wavelength spectrofluorometer was used to determine the concentration of intracellular Ca2 + in rat brains before and after the use of Antelope Capsule (Antelope horn, Scorpion, Panax notoginseng, Radix Astragali, etc.). Results: In the case of extracellular Ca2+ concentration of 1 mmol/L, the Ca2+ concentration in brain cells of normal rats was 32 0±83 nmol/L, 35 5± 48 nmol/L in sham-operated rats, and 10 10± in model rats. 118nmol/L, antelope capsule group was 930±84nmol/L, Qingkailing group was 910±59nmol/L. It is suggested that intracranial hemorrhage has severe Ca2+ overload in intracerebral brain cells of rats. The capsule can effectively reduce intracellular Ca2+ concentration in intracerebral hemorrhage rat brain cells.