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通过EMS诱变获得了1个可稳定遗传的番茄黄色柱头突变体,命名为ys(yellow stigma)。色素含量测定发现:同野生型相比,突变体ys柱头中的叶绿素和类胡萝卜素含量没有明显变化,但对香豆酸和柚皮素查尔酮含量显著高于野生型,推测呈黄色的柚皮素查尔酮的大量积累可能在黄色柱头形成过程中起着重要作用。此外,分析发现,突变体和野生型在柱头表面结构、柱头可授性及花粉活力方面没有显著差异。对突变体ys与野生型构建的F_1、F_2、BC_1以及BC_2群体的遗传分析发现,F_1和BC_2均为绿色柱头植株,F_2和BC_1群体绿色柱头植株与黄色柱头植株的比例符合3∶1和1∶1的分离比(P>0.05),表明黄色柱头突变受1对隐性基因控制,其遗传方式符合孟德尔遗传定律。
A mutagenized tomato yellow stigma mutant named ys (yellow stigma) was obtained by EMS mutagenesis. The content of chlorophyll and carotenoid in the stigma of mutant ys showed no significant change compared with wild type, but the content of chalcone and naringenin in chamomile and naringenin was significantly higher than that in wild type The large accumulation of naringenin chalcone may play an important role in the formation of yellow stigma. In addition, the analysis found no significant difference in stigma surface structure, stigma receptivity and pollen viability between mutant and wild-type. Genetic analysis of F_1, F_2, BC_1 and BC_2 populations constructed by mutant ys and wild type showed that both F_1 and BC_2 were green stigma. The ratio of green stigma and yellow stigma of F_2 and BC_1 populations was in the range of 3:1 and 1 : 1 (P> 0.05), indicating that the yellow stigma mutation was controlled by one pair of recessive genes and the inheritance mode accorded with Mendel ’s law of inheritance.