在光学显微镜下对胰岛细胞的鉴定,主要依赖于对这些细胞分泌颗粒的区别染色。自从 Lane 于1907年在豚鼠胰腺中描述了含有不同类型颗粒的 A 和 B 两种细胞以来,已有各种染色方法应用于胰岛细胞的区别染色。1931年,Bloom 首先采用 Mallory-Heiden-hain azan 染色法来染人的胰腺标本,在同一标本中能区别 A、B 和 D 细胞,这是胰岛细胞三色染色法的经典方法,据报导,它能对各种动物的三种胰岛细胞显示不同的颜 The identification of islet cells under an optical microscope relies primarily on the differential staining of the secretory granules of these cells. Since Lane described both types A and B cells containing different types of particles in a guinea pig pancreas in 1907, various staining methods have been applied to differential staining of islet cells. In 1931, Bloom first used the Mallory-Heiden-hain azan staining method to stain human pancreatic specimens to distinguish between A, B and D cells in the same specimen. This is the classical method of islet cell trichromatosis. It is reported that it Can be a variety of animal three islet cells show different colors