论文部分内容阅读
应用XM6逆转录病毒载体将人IL-2cDNA转入小鼠B16黑色素瘤细胞。丝裂霉素C处理的转染前后B16细胞作为瘤苗对小鼠进行免疫接种。结果显示，接种B16-IL-2细胞可明显排斥再移植黑色素瘤的生长。对脾淋巴细胞检测的结果表明，MLTR引起的淋巴细胞增殖与特异性CTL杀伤活性，以及NK、LAK活性与诱生的IL-2水平几项指标，B16-IL-2细胞免疫组均明显高于B16免疫组及对照组。提示转基因肿瘤细胞在体内分泌IL-2增强了机体的特异性与非特异性免疫功能。
Human IL-2 cDNA was transferred into mouse B16 melanoma cells using the XM6 retroviral vector. B16 cells were immunized as tumor vaccines before and after transfection with mitomycin C. The results showed that inoculation of B16-IL-2 cells significantly repels the growth of retransplanted melanoma. The results of splenic lymphocyte assays showed that lymphocyte proliferation induced by MLTR and specific CTL killing activity, as well as NK, LAK activity and induced IL-2 levels, were significantly higher in the B16-IL-2 cell immune group. In the B16 immune group and control group. It is suggested that the secretion of IL-2 in transgenic tumor cells enhances the specific and non-specific immune function of the body.