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目的观察喘可治注射液(CKZ)对慢性阻塞性肺疾病(COPD)大鼠肺组织IL-12/IL-12R/STAT4信号通路的影响。方法 50只SD大鼠随机分为正常组,模型组,CKZ高、中、低剂量(CKZ-H、CHZ-M、CKZ-L)组。除正常组外,各组复制COPD大鼠模型,并给予相应药物治疗,观察CKZ对COPD大鼠血清和肺泡灌洗液(BALF)中γ-干扰素(IFN-γ)、白细胞介素12(IL-12)、白细胞介素12受体(IL-12R)以及肺组织信号转导子和转录激活子4(STAT4)m RNA表达的影响。结果喘可治注射液各剂量组肺组织病理形态均有不同程度的改善,且降低COPD大鼠血清和BALF中IFN-γ、IL-12、IL-12R(除CKZ-L组BALF中IL-12R外)水平(P<0.05,P<0.01),可抑制大鼠肺组织STAT4 m RNA的表达(P<0.01,P<0.05)。结论喘可治注射液能一定程度改善COPD大鼠肺组织病理损害,其作用机制可能与其抑制IFN-γ、IL-12、IL-12R表达,干扰IL-12/IL-12R/STAT4信号通路对靶基因的激活,下调STAT4 m RNA表达,进而调节Th1/Th2分化失衡有关。
Objective To observe the effect of Chuankezhi Injection (CKZ) on IL-12 / IL-12R / STAT4 signaling pathway in lung tissue of chronic obstructive pulmonary disease (COPD) rats. Methods 50 Sprague-Dawley rats were randomly divided into normal group, model group, CKZ-H, CHZ-M and CKZ-L groups. In addition to the normal group, the COPD rat model was duplicated and the corresponding drug treatment was given to observe the effect of CKZ on the expression of IFN-γ, IL-12 (IL-6β) in serum and bronchoalveolar lavage fluid (BALF) IL-12, IL-12R, and lung tissue signal transducers and activator of transcription 4 (STAT4) m RNA expression. Results The pathological changes of lung tissue in each dose group of Chuankezhi injection were improved to some extent, and the levels of IFN-γ, IL-12 and IL-12R in serum and BALF of COPD rats were decreased 12R) (P <0.05, P <0.01), and inhibited STAT4 mRNA expression in lung tissue of rats (P <0.01, P <0.05). Conclusion Chuankezhi injection can improve lung tissue pathological damage to a certain extent in COPD rats, and its mechanism may be related to its inhibitory effect on the expression of IFN-γ, IL-12 and IL-12R and the interference of IL-12 / IL-12R / STAT4 signaling pathway Activation of target genes, downregulation of STAT4 mRNA expression, and thus regulation of imbalance of Th1 / Th2 differentiation.