论文部分内容阅读
目的:探讨关节通片对炎症关节模型的预防治疗作用、作用机制、及其急性毒性。方法:①采用角叉菜胶及甲醛大鼠踝关节炎症模型探讨关节通片对关节炎的防治作用;②采用佐剂性关节炎大鼠模型观察关节通片对关节液IL1β、TNFα及PGE2的影响;③最大耐受量法观察关节通片对小鼠的急性毒性。结果:关节通片能显著减轻角叉菜胶及甲醛致大鼠踝关节炎症的肿胀度(P<0.05～0.01);病理组织形态观察显示关节通片能减轻大鼠踝关节炎症时关节滑膜的充血、水肿,减少炎症细胞的浸润及关节囊内炎性渗出物;关节通片还能明显地降低模型大鼠关节液ILβ、TNFα及PGE2含量;小鼠一次灌服191.6%的关节通提取液的最大耐受量为40mL(76.64g)kg,为临床日用量的223倍。结论:关节通提取液对关节炎具有一定的预防及治疗作用,其作用与降低关节液中IL1β、TNFα及PGE2含量有关。
Objective: To investigate the preventive and therapeutic effects of arthrodesis on inflammatory joint models, its mechanism of action, and its acute toxicity. METHODS: 1 To investigate the prevention and treatment of arthritis on the arthritis model of carrageenan and formaldehyde rat ankle joint inflammation; 2 To observe the effect of arthroscopy on synovial fluid IL1β, TNFα and PGE2 in a rat model of adjuvant arthritis. Effect; 3 maximum tolerance method to observe the acute toxicity of the joints to mice. RESULTS: Arthrodesis could significantly reduce the degree of inflammation of the sacroiliac joints in rats caused by carrageenan and formaldehyde (P<0.05-0.01). Histopathologic observation showed that arthrodesis could reduce the synovial joint inflammation in rats. Congestion, edema, decreased inflammatory cell infiltration and inflammatory exudate in the joint capsule; arthrodesis can also significantly reduce the content of ILβ, TNFα, and PGE2 in synovial fluid of the model rat; the mice were fed 191.6% of joints at one time. The maximum tolerated amount of the extract was 40 mL (76.64 g) kg, which was 223 times the clinical daily dose. Conclusion: Arthritis extract has certain preventive and therapeutic effects on arthritis, and its effect is related to the decrease of IL1β, TNFα and PGE2 levels in synovial fluid.