论文部分内容阅读
目的观察白藜芦醇对食管癌细胞KYSE150的抑制及侵袭性能的影响。方法体外培养食管癌细胞KYSE150,用甲基噻唑蓝(MTT)法观察评价白藜芦醇对KYSE150的毒性,了解药物对细胞的抑制作用与时间、浓度的关系,并计算最高抑制率时间的IC50值;用Transwell侵袭小室观察KYSE150在不同实验条件下的侵袭能力变化。结果 MTT实验表明,白藜芦醇在0.16~100μg/mL浓度范围内对KYSE150的生长抑制作用具有浓度依赖性,24h抑制率达高峰。24h白藜芦醇对KYSE150的半数致死剂量:IC50=0.76μg/mL;白藜芦醇组与对照组相比,肿瘤细胞的侵袭能力下降,差异具有统计学意义。结论白藜芦醇对食管癌细胞KYSE150具有抑制作用,降低肿瘤细胞的侵袭性。
Objective To observe the effect of resveratrol on the inhibition and invasion of esophageal cancer cell KYSE150. Methods KYSE150 cells were cultured in vitro. Methyl thiazolyl tetrazolium (MTT) method was used to evaluate the toxicity of resveratrol to KYSE150. The relationship between the inhibitory effect and the time and concentration of resveratrol were evaluated. The IC50 The invasion ability of KYSE150 under different experimental conditions was observed with Transwell invasion chamber. Results MTT assay showed that resveratrol concentration-dependently inhibited the growth of KYSE150 in the concentration range of 0.16 ~ 100μg / mL, and reached the peak at 24h. The median lethal dose of 24 h resveratrol to KYSE150: IC50 = 0.76 μg / mL. The invasive ability of tumor cells in resveratrol group decreased compared with the control group, the difference was statistically significant. Conclusion Resveratrol has an inhibitory effect on KYSE150 esophageal cancer cells and reduces the invasiveness of tumor cells.