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Genetic transformation of embryogenic suspension cultures of sweetpotato cv. Lizixiang was conducted by using Agrobacterium tumefaciens strain A208SE harboring the binary vectors pROA93 with β-glucronidase(GUS)and neomycin phosphotransferase(NPTⅡ)genes. The results indicated that embryogenic suspension cultures precultured for 1 - 3 d were suitable for the transformation. The optimal cocultivation time was 4 - 5 d. The optimal concentration of kanamycin was 50-75 mg L-1 for suspension culture and 100 mg L-1 for embryogenic callus proliferation and plant regeneration. The optimal concentration of carbencillin was 100 mg L-1. Transgenic plants identified with GUS assays and PCR analyses were obtained.
Genetic transformation of embryogenic suspension cultures of sweetpotato cv. Lizixiang was conducted by using Agrobacterium tumefaciens strain A208SE harboring the binary vectors pROA93 with β-glucronidase (GUS) and neomycin phosphotransferase (NPTII) genes. The results indicated that embryogenic suspension cultures precultured for 1- The optimal concentration of kanamycin was 50-75 mg L-1 for suspension culture and 100 mg L-1 for embryogenic callus proliferation and plant regeneration. The optimal cocultivation time was 4-5 d. The optimal concentration of kanamycin was 50-75 mg L- concentration of carbencillin was 100 mg L-1. Transgenic plants identified with GUS assays and PCR analyzes were obtained.