为比较不同浓度神经髓鞘组织、神经髓鞘蛋白及其合成多肽作为抗原对多发性硬化 (MS )和正常人的淋巴细胞诱发增殖反应的量效关系。用HLA DR15亚型MS和正常对照外周血单个核细胞 ,体外培养并检测对不同浓度的人神经髓鞘、脱脂神经髓鞘、蛋白脂蛋白 (PLP )、碱性髓鞘蛋白 (MBP )、PLP178 191和MBP87 10 6多肽 6种抗原的T淋巴细胞增殖反应性。结果发现神经髓鞘或脱脂神经髓鞘作为抗原刺激T淋巴细胞增殖反应的最佳浓度为 5 0～ 15 0 μg/ml,PLP或MBP最佳浓度为 2 5～ 10 0 μg/ml,多肽最佳浓度为 2 0～ 5 0 μg/ml。神经髓鞘抗原刺激T淋巴细胞增殖反应大于单一蛋白分子 ,后者又大于多肽。MS的T细胞对神经髓鞘类抗原有特异性异常增殖反应。 To compare the dose-response relationship of different concentrations of myelin tissue, myelin sheath protein and their synthetic peptides as antigen on lymphocyte-induced proliferative response in multiple sclerosis (MS) and normal human. HLA DR15 subtype MS and normal control peripheral blood mononuclear cells were cultured and tested in vitro for different concentrations of human nerve myelin, skim myelin, protein lipoprotein (PLP), basic myelin protein (MBP), PLP178 191 and MBP87 10 6 polypeptide antigens T lymphocyte proliferation reactivity. The results showed that the optimal concentration of myelin sheath or defatted myelin sheath for antigen stimulation of T lymphocyte proliferation was 50 ~ 150 μg / ml, and the optimum concentration of PLP or MBP was 25 ~ 100 μg / ml. The best concentration is 20 ~ 50 μg / ml. Nerve myelin antigens stimulate T lymphocyte proliferation greater than single protein molecules, which is larger than the polypeptide. MS T cells have specific abnormal proliferative responses to myelin antigens.