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将２１只大鼠随机分成肿瘤坏死因子作用２４ｈ组、７２ｈ组和生理盐水对照组。实验组用重组人的肿瘤坏死因子α型（ｒｈ－ＴＮＦ－α）×１０￣５Ｕ·ｋｇ￣（－１）／ｄ持续静脉泵入，分别于２４ｈ．７２ｈ活杀，观察血液及心、肝、肺、肾、肠组织ＴＮＦ水平；过氧化物歧化酶（ＳＯＤ）活性；丙二醛及血浆内毒素含量；组织超弱发光和白细胞吞噬发光、血气、肝肾功能及血培养情况。结果：实验２４ｈ组血浆ＴＮＦ显著升高，各脏器功能无明显受损，血培养及内毒素测定阴性、白细胞吞噬功能无变化；但ＴＮＦ可使血浆及组织氧自由基和脂质过氧化产物增加，全血ＳＯＤ活性下降。
Twenty-one rats were randomly divided into 24h, 72h and saline control groups. Experimental group with recombinant human tumor necrosis factor α (rh-TNF-α) × 10 ~ 5U · kg ~ (-1) / d continuous intravenous infusion, respectively, at 24h. 72h were sacrificed to observe the level of TNF in the blood, heart, liver, lung, kidney and intestine; the activity of superoxide dismutase (SOD); the content of MDA and plasma endotoxin; Liver and kidney function and blood culture. Results: In the experimental group, the plasma TNF was significantly increased, the function of each organ was not significantly impaired, blood culture and endotoxin were negative, while the phagocytic function of leukocytes was unchanged. However, TNF and TNF-α produced significant changes in oxygen free radicals and lipid peroxidation products Increased, whole blood SOD activity decreased.