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目的:寻找内吗啡肽2(EM2)和μ阿片受体(MOR)在内脏感觉和运动调控过程中发挥作用的形态学证据。方法:(1)麦芽糖凝集素(WGA)示踪与免疫荧光组织化学染色技术相结合的多重标记方法;(2)用稀盐酸刺激胃,制作内脏痛动物模型;(3)免疫荧光组织化学多重染色方法。结果:(1)将WGA注入大鼠胃壁,在结状神经节(NG)内可见WGA标记神经元和WGA/EM2/MOR三标神经元;在孤束核(NTS)可见WGA跨节标记的纤维和终末以及WGA/EM2/MOR三标纤维和终末;在迷走神经运动背核(DMV)可见WGA逆标神经元和WGA/MOR双标神经元及其与EM2阳性纤维或终末形成的紧密接触。(2)将稀盐酸注入胃后,NG和NTS神经元内的FOS表达明显增多;在NG内可见EM2/FOS双标神经元;在NTS内可见EM2阳性纤维和终末与SP受体/FOS双标神经元形成紧密接触。结论:EM2和MOR可能影响内脏感觉和运动功能。
OBJECTIVE: To search for morphological evidence that endomorphin 2 (EM2) and mu opioid receptor (MOR) play roles in visceral sensation and motor regulation. Methods: (1) Multiple labeling method combining with WGA tracer and immunofluorescence histochemical staining technique; (2) Stimulating stomach with dilute hydrochloric acid to make animal model of visceral pain; (3) Immunofluorescence histochemistry Dyeing method. Results: (1) WGA-labeled neurons and WGA / EM2 / MOR triple-labeled neurons were found in the nodose ganglion (NG) Fibers and terminals, and WGA / EM2 / MOR triplets and terminals; WGA retrograde neurons and WGA / MOR double-labeled neurons visible in the dorsal vagal motor dorsal nucleus (DMV) and their formation with EM2 positive fibers or terminals Close contact. (2) After injecting dilute hydrochloric acid into the stomach, the expression of FOS in NG and NTS neurons increased obviously; EM2 / FOS double-labeled neurons were seen in NG; EM2 positive fibers and terminal and SP receptor / FOS Double-labeled neurons form close contact. Conclusion: EM2 and MOR may affect visceral sensation and motor function.