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1.用 PEG6000的不同浓度(30%,40%,50%)处理鸡红血细胞及小白鼠腹水型肝癌(Hca)细胞融合的结果,以40%对这两种细胞较为适宜,细胞融合的均数为2.9%.2.悬浮细胞的细胞融合是发生在 PEG 处理的10分钟后.3.PEG 加入细胞中时以边加边摇动细胞试管为好.
1. The results of fusion of chicken red blood cells and mouse ascites type Hca cells with different concentrations of PEG6000 (30%, 40%, 50%) are suitable for these two types of cells at 40%. The number was 2.9%. 2. The cell fusion of the suspension cells took place 10 minutes after the PEG treatment. 3. When the PEG was added to the cells, it was better to shake the cell tubes while adding.