Polar polymeric porous microspheres of ethyleneglycol dimethacrylate/ glycidylmethaerylate (EGPM) have been synthesized by suspension copolymerization in the presence of diluent with different nature and composition. The correlation of phase separation with crosslinking agent and diluent which are the key points of pore formation during polymerization has been discussed. The physicochemical properties and the porous structure of the microspheres are characterized by apparent density, specific surface area, skeleton density, pore radius distribution, solvent regain, pore volume, bed volume, degree of swelling and the scanning electron microscopy. The molecular weight vs elution volume calibration curve and the chromatogram of dextran separation of the microspheres are also presented. Such a comprehensive evaluation of EGPM shows that it possesses the characteristics of hydrophilic stationary phase for modern gel permeation chromatography. Further, the molecular weight polydispersity and the solute mass t Polar polymeric porous microspheres of ethyleneglycol dimethacrylate / glycidylmethaerylate (EGPM) have been synthesized by suspension copolymerization in the presence of diluent with different nature and composition. The correlation of phase separation with crosslinking agent and diluent which are the key points of pore formation during polymerization has has discussed. The physicochemical properties and the porous structure of the microspheres are characterized by apparent density, specific surface area, skeleton density, pore radius distribution, solvent regain, pore volume, bed volume, degree of swelling and the scanning electron microscopy. weight versus elution volume calibration curve and the chromatogram of dextran separation of the microspheres are also presented. Such a comprehensive evaluation of EGPM shows that it possesses the characteristics of hydrophilic stationary phase for modern gel permeation chromatography. Further, the molecular weight polydispersity and the solute mass t