论文部分内容阅读
冷冻在生物学上有两种截然不同的作用:1.促使细胞坏死;2.维持细胞生命。基于此作用而可达到保存组织或破坏组织的目的。影响细胞生死的因素,主要为1.细胞的冷却温度与速度;2.复温速度;3.细胞或组织对低温侵袭的感受性。一般细胞和组织破坏的临界温度为-5℃——-50℃,特别在-20℃左右,Mazur等谓以1,000℃/分极快速度冷却与100℃/分急速复温,可以保存细胞 Schrott提出,倘以100℃/分以下速度缓慢冷冻,即冷冻干燥或加入甘油等吸水物质。使细胞内游离水分丧失,即使温度降至-200℃细胞仍能生存而不破坏。相反,以100℃/分快速冷却与10℃/分缓慢复温,则可使细胞或组织破坏最大。
Freezing has two distinctly different biological roles: 1. It promotes cell necrosis; 2. It maintains cell life. Based on this effect, the purpose of keeping the tissue or destroying the tissue can be achieved. The factors affecting cell life and death are 1. cooling temperature and speed of cells; 2. rewarming speed; 3. susceptibility of cells or tissues to cold invasion. The critical temperature for cell and tissue destruction in general is -5°C to -50°C, especially around -20°C. Mazur et al. stated that cooling at 1,000°C/min and rapid rewarming at 100°C/min can preserve cells Schrott It is proposed that if it is slowly frozen at a rate of 100°C/min or less, it is freeze-dried or a water-absorbent substance such as glycerin is added. Loss of intracellular free water, even if the temperature dropped to -200 °C cells can survive without destruction. In contrast, rapid rewarming at 100°C/min and 10°C/min rewarming can maximize cell or tissue destruction.