论文部分内容阅读
目的:建立与人类早产儿脑室周围白质软化(PVL)病理相似的可靠动物模型。方法:144只2日龄(P2)SD新生大鼠,体重6~9 g,雌雄不拘,随机分成正常对照组(48只),假手术组(48只)、缺氧缺血性脑损伤(HIBD)组(48只)。P2新生大鼠麻醉后经结扎左侧颈总动脉及8%浓度的氧气缺氧4 h制作HIBD组。假手术组麻醉后分离左侧颈总动脉不结扎不缺氧。正常对照组未予任何处理。缺氧缺血后3天、1周、2周、4周时,各组大鼠分别麻醉后予4℃生理盐水及4%多聚甲醛心脏灌注,脑组织于4%多聚甲醛中后固定,观察脑形态学改变,脑组织石蜡切片行HE染色,病理评分。结果:HIBD组可见白质水肿、结构疏松、左侧纹状体梗死灶形成、病灶内胶质细胞浸润,丘脑及海马可见细胞凋亡。5日龄(P5)大鼠病理评分:HIBD组与正常对照组、假手术组差异有统计学意义(P<0.01);9日龄(P9)大鼠病理评分:HIBD组与正常对照组、假手术组差异有统计学意义(P<0.05);16日龄(P16)大鼠病理评分:HIBD组与正常对照组、假手术组差异有统计学意义(P<0.01);30日龄(P30)大鼠病理评分:HIBD组与正常对照组、假手术组差异有统计学意义(P<0.01)。HIBD组评分均高于其他组,且呈上升趋势。结论:通过对P2新生大鼠麻醉后经结扎左侧颈总动脉及8%浓度的氧气缺氧4 h,成功建立与人类早产儿PVL病理变化相似的动物模型。
Objective: To establish a reliable animal model of periventricular leukomalacia (PVL) pathology in human premature infants. METHODS: One hundred and fourteen 2-day-old (SD) SD neonatal rats weighing 6 to 9 g were randomly divided into normal control group (48), sham operation group (48), hypoxic-ischemic brain injury HIBD) group (48). P2 neonatal rats anesthetized by left common carotid artery ligation and 8% oxygen for 4 h hypoxia produced HIBD group. Sham-operation group after anesthesia, left common carotid artery was ligated without hypoxia. Normal control group without any treatment. After hypoxia and ischemia for 3 days, 1 week, 2 weeks and 4 weeks, the rats in each group were anesthetized and then perfused with 4% paraformaldehyde and 4% normal saline. The brain tissues were fixed in 4% paraformaldehyde The changes of brain morphology were observed. The brain tissue paraffin sections were stained with HE and pathological score. Results: HIBD group showed white matter edema, loose structure, left striatum infarction formation, glial cell infiltration in lesion, apoptosis in thalamus and hippocampus. The pathological score of rats of 5-day-old (P5): HIBD group and normal control group, the sham group had significant difference (P <0.01); The pathological score of 9-day- (P <0.05); Pathological score of 16-day-old rats (P16): There was significant difference between HIBD group and normal control group, sham operation group (P <0.01) P30) pathological score of rats: HIBD group and normal control group, sham operation group difference was statistically significant (P <0.01). HIBD group scores were higher than the other groups, and the upward trend. Conclusion: The animal models of PVL pathology similar to human premature infants were successfully established by ligating left carotid artery and 8% oxygen for 4 h after anesthesia in P2 neonatal rats.