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目的探讨N-乙酰半胱氨酸(NAC)对二氧化硅所致大鼠肺纤维化血清及肺组织中丙二醛(MDA)的影响。方法取健康、雄性Wistar大鼠90只,随机分为3组:染尘组:采用经非暴露气管注入法,气管内注入无菌SiO2粉尘悬液(50 mg/ml)1 ml建立矽肺模型;4周后再次予以染尘1次。NAC预防治疗组:模型制备同染尘组,于染尘前1周及此后每日予以NAC[500 mg/(kg.d)]灌胃;对照组:同样条件下向气管内注入等量生理盐水。3组动物于实验第8周处死,取肺组织标本行病理学观察、测定肺组织及血清中MDA水平。结果染尘组及预防治疗组MDA含量明显高于对照组;预防治疗组MDA的含量明显低于染尘组,差异有统计学意义(P<0.01)。结论 NAC可降低SiO2所致肺纤维化大鼠MDA的产生,抑制肺纤维化的发展,减轻对肺组织的损伤。
Objective To investigate the effect of NAC on malondialdehyde (MDA) in serum and lung of rats with pulmonary fibrosis induced by silica. Methods Totally 90 healthy and male Wistar rats were randomly divided into 3 groups: Dust-exposed group: Silicosis model was established by injecting 1 ml of sterile SiO2 dust suspension (50 mg / ml) intratracheally via non-exposed tracheal injection; 4 weeks after being dyed once again. NAC prophylaxis treatment group: the model was prepared with the same group of dust, one week before dyed and then daily with NAC [500 mg / (kg · d)]; the control group: the same conditions were injected into the trachea by the same amount of physiology brine. Three groups of animals were sacrificed on the 8th week of the experiment. Pathological examination was performed on the lung tissue samples to determine the level of MDA in lung tissue and serum. Results The content of MDA in the dust-treated group and the prophylaxis-treated group was significantly higher than that in the control group. The content of MDA in the preventive treatment group was significantly lower than that in the dust-exposed group (P <0.01). Conclusion NAC can reduce the production of MDA in pulmonary fibrosis rats induced by SiO2, inhibit the development of pulmonary fibrosis, and reduce the damage of lung tissue.