本文介绍了一个简易、快速、微量甲胎旦白(AFP)放射免疫测定一聚乙二醇(PEGMW12000)沉淀法。分离抗原-抗体复合物的PEG最终浓度是7.5％,各试剂量均为50μl,保温是37℃30分钟,离心是3000rpm/分,15分钟。弃上清,测沉淀中~(125)I-AFP放射量。每次测定都做标准曲线,以ng/ml表示AFP量,方法简易,灵敏,重复性好、适于临床化验室常规操作。 This article describes a simple, rapid, trace AFP radioimmunoassay (PEGMW12000) precipitation method. The final concentration of PEG for separating the antigen-antibody complexes was 7.5%, the amount of each reagent was 50 μl, the incubation was 37°C for 30 minutes, and the centrifugation was 3000 rpm/minute for 15 minutes. The supernatant was discarded and the amount of ~(125)I-AFP in the precipitate was measured. A standard curve was used for each determination, and the AFP amount was expressed in ng/ml. The method was simple, sensitive, and reproducible, and was suitable for routine operation in clinical laboratories.