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建立了适合环孢素A、硫唑嘌呤、强的松三联用药系统分离的胶束电动毛细管色谱（MECC）方法,考察了运行缓冲液体系中SDS浓度、乙腈含量及电渗流促进剂牛磺酸（FX）比例对电泳分离方法耐用性的影响。MECC优化分离系统为：毛细管50cm×50μm×50μmuncoated（Bio-Rad）,缓冲液组成：45mmol／LSDS的背景电解质溶液（50mmol／L磷酸二氯钠,12.5mmol／L硼砂,PH8.25）-乙腈-FX（50：50：25）,操作电压18kV（＋）→（-）,进样量10psi×s,柱上检测UV200nm,毛细管柱温25℃。器官移植手术常用的环孢素A、硫唑嘌呤和强的松三联用药在10min之内获得了理想的分离。
A micellar electrokinetic capillary chromatography (MECC) method was established for the separation of cyclosporin A, azathioprine and prednisone. The effects of SDS concentration, acetonitrile concentration and electrosurgery accelerator taurine (FX) ratio on the durability of electrophoretic separation methods. The MECC optimized separation system consisted of a capillary 50 cm × 50 μm × 50 μmuncoated (Bio-Rad) buffer solution consisting of a background electrolyte solution of 45 mmol / L DSDS (50 mmol / L sodium phosphate dibasic, 12.5 mmol / L borax, -FX (50:50:25), operating voltage 18kV (+) → (-), injection volume 10psi × s, column detection UV200nm, capillary column temperature 25 ℃. Cyclosporin A, azathioprine and prednisone, commonly used in organ transplant surgery, achieved the ideal separation within 10 minutes.