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目的 观察不同剂量维生素C(VitC)对大鼠肾结石模型体内活性氧 (ROS)的影响及VitC、ROS与肾结石形成的关系。 方法 用乙二醇诱导Wistar大鼠产生肾草酸钙结石。 30只大鼠分为正常组 ,成石组 ,治疗组 (分三个不同剂量 ,VitC 2 5、10 0、4 0 0mg·kg-1·d-1) ,喂养 15d。测定血和右肾组织中丙二醛 (MDA)、过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶(GSH Px)含量 ;左肾冰冻切片 ,偏光显微镜观察结晶情况。 结果 成石组血和组织中的MDA分别为 (43.89± 5 .10 )nmol/ml和 (6 .2 0± 2 .0 0 )nmol/gpr,较正常组增高 ,差别有显著性意义 (P <0 .0 1和P <0 .0 0 1) ,而抗氧化酶显著降低 (P <0 .0 1和P <0 .0 0 1)。VitC 4 0 0mg·kg-1·d-1治疗组血MDA(30 .80± 4 .6 9)nmol/ml,较成石组降低 (P <0 .0 5 ) ,与正常组比较差别无显著性意义 (P >0 .0 5 ) ,血SOD较成石组降低 (P <0 .0 5 ) ,血CAT、GSH Px较成石组升高 (P <0 .0 1) ;VitC 4 0 0mg·kg-1·d-1治疗组肾组织中MDA(11.96± 2 .4 4 )nmol/gpr ,较成石组升高 (P <0 .0 5 ) ,SOD较成石组升高 (P <0 .0 5 ) ,CAT、GSH Px较成石组有所降低。肾组织晶体形成与VitC剂量呈正相关 (r =0 .6 6 85 ,P <0 .0 1)。 结论 高草酸尿可使机体活性?
Objective To investigate the effects of different doses of vitamin C on reactive oxygen species (ROS) in rat kidney stone and the relationship between VitC and ROS and the formation of kidney stones. Methods Wistar rats were induced with calcium oxalate stones by ethylene glycol. Thirty rats were divided into normal group, Chengshu group and treatment group (three different doses of VitC 2, 10, 400mg x kg (-1) d-1) for 15 days. The content of malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH Px) in the blood and the right kidney were measured. Polarizing microscope to observe the crystallization. Results The levels of MDA in blood and tissues of Chengshi Formation were (43.89 ± 5.10) nmol / ml and (6.2 ± 2.000 nmol / gpr), respectively, which were significantly higher than those in normal group (P <0.01 and P <0.001), but the antioxidant enzymes decreased significantly (P <0.01 and P <0.01). Compared with the normal group, the serum MDA level of VitC 400 mg · kg-1 · d-1 treatment group (30 .80 ± 4.69) nmol / ml was lower than that of the control group (P <0.05) Significant significance (P> 0.05), blood SOD decreased compared with Chengshi group (P <0.05), blood CAT, GSH Px increased compared with Chengshi group (P <0.01); VitC 4 The level of MDA (11.96 ± 2.44 nmol / gpr) in the 0 mg · kg-1 · d-1 treatment group was significantly higher than that in the chengshi group (P <0.05) (P <0. 05), CAT, GSH Px decreased compared with the stone group. Kidney crystal formation and VitC dose was positively correlated (r = 0.6685, P <0.01). Conclusion High oxalate urine can make the body active?