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Objective:To observe the effect of laparoscopic peritoneal dialysis (PD)catheterization on stress response. Methods: The clinical data of 60 patients with stage 5 chronic kidney disease treated in our hospital from March 2016 to October 2019 were analyzed retrospectively. According to the different operation methods, they were divided into two groups. 34 patients who received open PD catheterization were included in the control group, and 26 patients who received laparoscopic PD catheterization were used as the observation group. The intraoperative indexes, C-reactive protein (CRP), procalcitonin (PCT) levels and complications were compared between the two groups. Results: In the observation group, the bleeding volume was (16.27±4.13) mL, less than that in the control group, the operation time was (48.93±10.16) min, shorter than that in the control group, the incidence of catheter displacement was lower than that in the con-trol group, the difference was statistically significant (P<0.05). There was no significant difference in the levels of CRP and PCT between the two groups (P>0.05);the levels of CRP (47.25±16.91) mg/L and PCT (0.07±0.01)μg/L in the observation group were lower than those in the control group (P<0.05). Conclusion:Laparoscopic PD catheteri-zation in the treatment of end-stage renal disease has the advantages of small trauma, mild stress response and quick postoperative recovery, and it can reduce the occurrence of catheter displacement and the risk of PD catheterization.