以原发肝癌及肝癌7402细胞株DNA转染NIH/3T3得到的转化细胞与材料,经~(35)S-甲硫氨酸参入,应用放射免疫吸附,聚丙烯酰胺凝胶电泳技术,发现原发性肝癌的转化细胞株中N-ras基因的表达产物p21明显高于非转化的细胞。7402细胞株和7402转化的细胞株可能存在有二个ras基因,一个是ras~N;另一个是ras~H。本文结果为证明N-ras是人肝癌的一种转化基因提供了证据。 The transformed cells and materials obtained by transfecting NIH/3T3 with DNA of primary liver cancer and hepatoma 7402 cell line were infused with ~(35)S-methionine, applied radioimmunosorbent, polyacrylamide gel electrophoresis, and discovered the original The expression product of p21 of the N-ras gene in the transformed cell line of hepatocellular carcinoma was significantly higher than that of non-transformed cells. The 7402 cell line and the 7402 transformed cell line may have two ras genes, one is ras~N; the other is ras~H. This article provides evidence that N-ras is a transforming gene for human liver cancer.