建立了高效液相色谱法同时测定葛根芩连片中的葛根素、盐酸小檗碱和黄芩苷。采用C_(18)色谱柱,以甲醇-0.2%磷酸为流动相,梯度洗脱,双波长检测(葛根素为250 nm,盐酸小檗碱和黄芩苷为280 nm)。葛根素、盐酸小檗碱和黄芩苷分别在0.02～0.40μg、0.01～0.20μg和0.01～0.20μg范围内线性关系良好,平均回收率分别为99.2%、97.1%和97.9%,RSD分别为1.7%、2.1%和1.9%。 A high performance liquid chromatography (HPLC) was developed for simultaneous determination of puerarin, berberine and baicalin in Puerarin. The mobile phase was eluted with C_ (18) column and eluted with methanol-0.2% phosphoric acid with gradient elution and detected by dual wavelength (250 nm for puerarin, 280 nm for berberine hydrochloride and baicalin). Puerarin, berberine hydrochloride and baicalin showed good linearity in the range of 0.02 ~ 0.40μg, 0.01 ~ 0.20μg and 0.01 ~ 0.20μg, with the average recoveries of 99.2%, 97.1% and 97.9%, respectively, with RSDs of 1.7 %, 2.1% and 1.9%.