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目的 :建立适应大批量样品快速自动分析的阿昔洛韦血药浓度HPLC测定方法。方法 :应用自制自动磁力定时切换装置。分析柱采用WatersNova PakC18柱 (15 0mm× 3.9mm ,4μm) ,分析流动相为甲醇 - 0 .0 1mol·L-1磷酸氢二钾缓冲液 =3∶97(v/v) ,流速 1ml·min-1;预处理柱为 μBondapakC18柱 (5 0mm× 4.6mm ,37～ 5 0 μm) ,预处理流动相 (PMP)为含0 .0 1mol·L-1庚烷磺酸钠的磷酸缓冲液 ,流速 1ml·min-1;检测波长 2 5 4nm。结果 :阿昔洛韦血药浓度在 40～ 12 80ng·ml-1线性良好(r =0 .9998) ,最低检测限约为 2ng(S/N≥ 3) ,最低血浆药物检测浓度为 8ng·ml-1;绝对和相对回收率分别为 (91.2± 8.3) %和 (98.3± 5 .5 ) % ;日内及日间精密度都小于 10 %。结论 :方法简便、快速、灵敏、准确 ,适于大批量血样的分析。
Objective: To establish an HPLC method for the determination of acyclovir in plasma by rapid and automatic analysis of large quantities of samples. Methods: Self-made automatic magnetic timing switching device. The analytical column was equipped with a Waters Nova Pak C18 column (150 mm × 3.9 mm, 4 μm). The mobile phase was methanol-0.1mol·L -1 potassium phosphate dibasic buffer = 3:97 (v / v) and the flow rate was 1 ml · min -1. The pretreatment column consisted of μBondapak C18 column (50 mm × 4.6 mm, 37 ~ 50 μm). The pretreatment mobile phase (PMP) was phosphate buffer containing 0.01 mol·L -1 sodium heptane sulfonate, Flow rate 1ml · min-1; detection wavelength 254nm. Results: The linear range of aciclovir concentration was 40-1280ng · ml-1 (r = 0.9998), the lowest detection limit was 2ng (S / N≥3), the lowest concentration of plasma drug was 8ng · ml-1; the absolute and relative recoveries were (91.2 ± 8.3)% and (98.3 ± 5.5)%, respectively; the intra- and inter-day precision was less than 10%. Conclusion: The method is simple, rapid, sensitive and accurate and suitable for the analysis of large quantities of blood samples.