花粉和花药培养研究对遗传学和突变研究有重要意义,在植物育种和作物改良上又有各式各样的应用潜力。从作者的经验来说,尼施及其同事发展起来的花粉培养方法,用于一系列植物种成功,但比花药培养要困难得多,而且有些步骤不可靠,效率差。此外,植株产量一般也比用花药培养方法的低。作者这里介绍另外一条培养途径,它保持了花药培养简单、可靠的优点,而又不需要机械分裂或外科解剖花药。作者介绍的新途径是根据这一事实,即许多种植物的花药接种后很快裂开,如果花药浮在塑料培皿中液体培养基的浅层上,花粉就会“脱落”到培养基中。从培皿中除掉花药,脱落 Research on pollen and anther culture is of great importance to genetics and mutation research. There are various potential applications for plant breeding and crop improvement. In the author’s experience, the method of pollen cultivation developed by Niš and colleagues is a success for a range of plant species but much more difficult than anther culture, with some steps unreliable and inefficient. In addition, plant yields are generally lower than those used for anther culture. The authors here introduce another approach to culture that maintains the simplicity and reliability of anther culture without the need for mechanical splitting or surgical dissection of anthers. The new approach introduced by the authors is based on the fact that the anthers of many species quickly split after anther inoculation, and the pollen “peels” into the medium if the anthers float on the shallow layer of liquid medium in plastic petri dishes . Remove the anther from petri dish and peel off