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DNA加合物检测方法的建立为致癌物的人群危险性评价提供了一个有力手段,但应用于人群生物监测时所面临的一个主要问题是如何非创伤性获得靶组织的DNA样本。本文报道了分离脱落于尿中的微量膀胱上皮细胞DNA及用~(32)P-后标记法检测DNA加合物的方法。实验动物选用狗,将4-氨基联苯(ABP)按300 mg/ml溶于三辛烷偶姻(trioctanion),然后放人琼脂胶囊内以5 mg/kg体重经口染毒,每周5次,共染毒两周。每天在冰冷条件下收集24小时全尿,每个尿样都要进行镜检
The establishment of DNA adduct detection method provides a powerful tool for assessing the risk of carcinogens. However, one of the major problems when applying it to biological monitoring is how to obtain DNA samples of target tissues noninvasively. This paper reports the isolation of DNA from human urinary bladder epithelial cells and the detection of DNA adducts by ~ (32) P-post-labeling. Dogs were selected as experimental animals and 4-aminobiphenyl (ABP) was dissolved in trioctanion at 300 mg / ml and then inoculated into agar capsules to be orally administered at a dose of 5 mg / kg body weight per week for 5 Times, co-injection two weeks. Urine collected 24 hours a day in all conditions, urine, microscopic examination of each urine