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目的：观察大鼠多发伤合并休克早期肺脏中细胞凋亡的发生情况。方法：采用ＤＮＡ琼脂糖凝胶电泳、原位末端标记法（ＩＳＥＬ），在光镜和透射电镜下检测细胞凋亡，并测定ＤＮＡ片段百分率（ａｐ％）作定量分析。结果：检测到细胞凋亡所特有的ＤＮＡ电泳梯形带，ＩＳＥＬ法亦显示出阳性结果。复苏后６小时，ａｐ％随创伤程度的加重而升高，且与肺通透指数成正相关（ｒ＝０．６２１，Ｐ＜０．００１）。在６处创伤合并休克组，复苏后１小时ａｐ％已显著升高（５．３％±０．６％，Ｐ＜０．０１），３小时即达顶峰（３１．０％±３．０％，Ｐ＜０．０１），以后逐渐下降。形态学显示，细胞凋亡发生于肺脏中的多种细胞（包括肺泡上皮细胞、血管内皮细胞和中性粒细胞）。结论：多发伤合并休克早期大鼠肺脏中发生细胞凋亡，且细胞凋亡可能在早期的肺功能损伤中发挥作用
Objective: To observe the occurrence of apoptosis in the lungs of rats with multiple traumatic injuries and early shock. Methods: Apoptosis was detected by DNA agarose gel electrophoresis and in situ end labeling (ISEL). The percentage of DNA fragments was determined by light microscope and transmission electron microscope. Results: The DNA ladder characteristic of apoptosis was detected, ISEL method also showed positive results. At 6 hours after recovery, ap% increased with the severity of trauma and positively correlated with pulmonary permeability index (r = 0.621, P <0.001). At 6 wounds with shock, ap% increased significantly at 1 hour after resuscitation (5.3% ± 0.6%, P <0.01) and peaked at 3 hours (31.0% ± 3.0 %, P <0.01), then gradually decreased. Morphology shows that apoptosis occurs in a variety of cells in the lung (including alveolar epithelial cells, vascular endothelial cells, and neutrophils). CONCLUSION: Apoptosis occurs in the lungs of rats with multiple traumatic shock and early shock, and apoptosis may play a role in early lung injury