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目的 :探讨两种孕激素受体亚型 (hPR A和hPR B)在子宫肌瘤发生、发展中的意义 ,为临床药物治疗子宫肌瘤提供理论依据。方法 :选取 30例因子宫肌瘤行全子宫切除术的标本 ,取肌瘤组织和正常肌层组织分别制成石蜡切片和冰冻标本。前者经免疫组化定位研究孕激素受体亚型 ;后者经蛋白质提取和Western印迹定量研究孕激素受体亚型的蛋白表达 ;从冰冻组织中提取RNA ,通过逆转录 多聚酶链反应 ,半定量研究孕激素受体亚型的mRNA表达。结果 :(1)免疫组化显示子宫肌瘤和正常肌层细胞的细胞核中 ,可见hPR阳性颗粒 ;(2 )蛋白定量中hPR (A +B)和hPR A在子宫肌瘤的表达均高于正常肌层 ;子宫肌瘤和正常肌层中 ,hPR B表达均明显高于hPR A ;(3)hPR A、hPR B和hPR (A +B)的mRNA在肌瘤的表达量均高于周围正常肌层 ;(4 )无论在子宫肌瘤还是在正常肌层组织中 ,hPR A与hPR B的mRNA表达量差异无显著性 ,与A、B亚型受体蛋白在子宫肌瘤和正常肌层的分布差异有显著性的情况不平行 ,提示可能存在转录后调控的差异。结论 :hPR A、hPR B为核受体 ;A亚型蛋白表达在子宫肌瘤高于正常肌层 ;A、B亚型的mRNA在子宫肌瘤表达均高于正常肌层 ;孕激素受体亚型 ,特别是hPR A与子宫肌瘤的发生有关
Objective: To explore the significance of the two progesterone receptor subtypes (hPR A and hPR B) in the occurrence and development of uterine fibroids and to provide a theoretical basis for the clinical drug treatment of uterine fibroids. Methods: Thirty cases of uterine fibroids were selected for hysterectomy. Paraffin sections and frozen specimens were taken from myoma and normal myometrium respectively. The former is localized by immunohistochemistry to study progesterone receptor subtypes; the latter is used to quantitatively study the protein expression of progesterone receptor subtypes by protein extraction and Western blotting; RNA is extracted from frozen tissues and semi-quantitatively by reverse transcriptase-polymerase chain reaction Study progesterone receptor subtype mRNA expression. Results: (1) Immunohistochemistry showed hPR-positive granules in the nucleus of uterine leiomyoma and normal myometrial cells; (2) The expression of hPR (A + B) and hPR A in uterine fibroids was higher than (3) The mRNA expression of hPR A, hPR B and hPR (A + B) in fibroids were higher than that of the surrounding tissues in normal myometrium, uterine fibroids and normal myometrium (4) The mRNA expression levels of hPR A and hPR B had no significant difference in both hysteromyoma and normal muscularis mucosa, but not with A and B receptor proteins in uterine leiomyoma and normal muscle The differences in the distribution of the layers were not parallel, suggesting that there may be differences in post-transcriptional regulation. Conclusion: hPR A and hPR B are nuclear receptors. The expression of subtype A protein is higher than normal myometrium in uterine fibroids. The mRNA expression of subtype A and B in uterine fibroids is higher than that in normal myometrium. Subtypes, especially hPR A, are associated with the development of uterine fibroids