论文部分内容阅读
Most important agronomic and quality traits of crops are quantitative in nature.The genetic variations in such traits are usually controlled by sets of genes called quantitative trait loci (QTLs),and the interactions between QTLs and the environment.It is crucial to understand the genetic architecture of complex traits to design efficient strategies for plant breeding.In the present study,a new experimental design and the corresponding statistical method are presented for QTL mapping.The proposed mapping population is composed of double backcross populations derived from backcrossing both homozygous parents to DH (double haploid) or RI (recombinant inbreeding) lines separately.Such an immortal mapping population allows for across-environment replications,and can be used to estimate dominance effects,epistatic effects,and QTL-environment interactions,remedying the drawbacks of a single backcross population.In this method,the mixed linear model approach is used to estimate the positions of QTLs and their various effects including the QTL additive,dominance,and epistatic effects,and QTL-environment interaction effects (QE).Monte Carlo simulations were conducted to investigate the performance of the proposed method and to assess the accuracy and efficiency of its estimations.The results showed that the proposed method could estimate the positions and the genetic effects of QTLs with high efficiency.
Most important agronomic and quality traits of crops are quantitative in nature. The genetic variations in such traits are usually controlled by sets of genes called quantitative trait loci (QTLs), and the interactions between QTLs and the environment. It is crucial to understand the genetic architecture of complex traits to design efficient strategies for plant breeding. a new experimental design and the corresponding statistical method are presented for QTL mapping. proposed mapping population is composed of double back cross populations derived from back crossross both homozygous parents to DH double haploid or RI (recombinant inbreeding) lines separately. Use an immortal mapping population allows for across-environment replications, and can be used to estimate dominance effects, epistatic effects, and QTL-environment interactions, remedying the drawbacks of a single back cross population.In this method, the mixed linear model approach is used to estimate the positions of QTLs and their various effects including the QTL additive, dominance, and epistatic effects, and QTL-environment interaction effects (QE). Monte Carlo simulations were conducted to investigate the performance of the proposed method and to assess the accuracy and efficiency of its estimations The results showed that the proposed method could estimate the positions and the genetic effects of QTLs with high efficiency.