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目的:研究ERβ基因表达及多态性与胃癌发生发展的相关性。方法:以100例原发性胃癌为实验组,90例胃溃疡为对照组,免疫组织化学SP法检测ERβ蛋白,限制性酶切法检测ERβ基因CA重复序列多态性和G1082A多态性。结果:ERβ实验组和对照组阳性率分别为68.0%和23.3%,差异有统计学意义(P<0.01),并且分化程度低、浸润深、有淋巴结转移和5年死亡者的ERβ阳性率分别高于分化程度高、浸润浅、无淋巴结转移和5年生存者,且Ⅰ、Ⅱ期低于Ⅲ期(P<0.01,P<0.05),而与初复发、年龄、肿瘤大小和组织学类型无关(P>0.05)。ERβ基因CA重复序列对照组和实验组基因型分布均符合Hardy-Weinberg平衡(P>0.05),两组基因型SS、SL、LL分布和等位基因S、L频率的比较,差异无统计学意义(P>0.05)。ERβG1082A多态性中,RsaⅠ和AluⅠ酶切分析:实验组和对照组均符合Hardy-Weinberg遗传平衡定律(P>0.05)。两组基因型rr、Rr、RR分布和等位基因r、R和a、A频率的比较差异无统计学意义(P>0.05);而实验组aa基因型频率与对照组相比,差异有统计学意义(P<0.05),且该基因型与肿瘤的临床分期、分化程度、浸润深度、转移情况、5年生存情况密切相关(P<0.05),与肿瘤初复发、患者年龄、肿瘤大小、组织学类型无关(P>0.05),而等位基因频率中仅a/A与肿瘤的分化程度、浸润深度密切相关(P<0.01)。结论:ERβ与胃癌的发生、分化程度、浸润深度、转移及5年存活率密切相关,并且ERβ的aa基因型和等位基因a/A与胃癌的关系极大。
Objective: To study the relationship between ERβ gene expression and polymorphism and the occurrence and development of gastric cancer. Methods: 100 cases of primary gastric cancer as experimental group and 90 cases of gastric ulcer as control group. Immunohistochemical SP method was used to detect ERβ protein. Restriction endonuclease method was used to detect CA repeat polymorphism and G1082A polymorphism of ERβ gene. Results: The positive rates of ERβ in the experimental and control groups were 68.0% and 23.3%, respectively, with significant difference (P <0.01), and the positive rate of ERβ in patients with low differentiation, deep infiltration, lymph node metastasis and 5-year death (P <0.01, P <0.05), but not with stage Ⅰ, stage Ⅱ and stage Ⅲ (P <0.01, P <0.05), but not with stage Ⅰ, stage Ⅱ and stage Ⅲ Not related (P> 0.05). The distribution of genotypes of ERβ gene CA repeats in control and experimental groups were in accordance with Hardy-Weinberg equilibrium (P> 0.05). There was no significant difference in the distribution of SS, SL and LL genotypes and S and L allele frequencies Significance (P> 0.05). ERβG1082A polymorphism, Rsa Ⅰ and Alu Ⅰ digestion analysis: experimental group and control group were in line with the Hardy-Weinberg law of genetic balance (P> 0.05). There was no significant difference in the distribution of rr, Rr and RR between the two genotypes and the r, r and a, A allele frequencies (P> 0.05). However, the frequency of aa genotype in the experimental group was significantly higher than that in the control group (P <0.05), and the genotype was closely related to the clinical stage, differentiation, depth of invasion, metastasis and 5-year survival (P <0.05) , But not histological type (P> 0.05). Only a / A in allele frequency was closely related to tumor differentiation and invasion depth (P <0.01). CONCLUSIONS: ERβ is closely related to the occurrence, differentiation, depth of invasion, metastasis and 5-year survival rate of gastric cancer. The aa genotype and allele a / A of ERβ have a great relationship with gastric cancer.