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目的对大鼠NR2B基因核心启动子区进行单核苷酸多态性(single nucleotide polymorphism,SNP)筛查及分析。方法提取16只健康SD大鼠海马组织基因组DNA,聚合酶链式反应(PCR)扩增NR2B基因核心启动子区序列和SNP库已报道的rs8169392所在区域序列,结合DNA测序方法进行SNP筛查和验证。结果在大鼠NR2B核心启动子区705bp中,发现1个新的SNP位点,为-217位C/T多态;本研究中未筛查到NCBI-SNP库报道的rs8169392。结论 -217位C/T多态的发现为NR2B基因多态性数据库提供了新信息。
Objective To screen and analyze single nucleotide polymorphism (SNP) of NR2B gene core promoter region in rats. Methods Genomic DNA was extracted from the hippocampus of 16 healthy SD rats. The region of NR2B core promoter region amplified by polymerase chain reaction (PCR) and rs8169392 region reported by SNP library were amplified by polymerase chain reaction (PCR). SNPs were screened by DNA sequencing. verification. Results A new SNP site was found at 705bp in the NR2B core promoter region of rat, with a -217 C / T polymorphism. In the present study, rs8169392 was not reported in the NCBI-SNP database. Conclusion The discovery of the -217 C / T polymorphism provides new information for the NR2B gene polymorphism database.