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在含有1毫克/升吲哚乙酸和2毫克/升6-苄基腺嘌呤(BA)的MS-Nitsch培养基上,从“苏母娜”葡萄(Vitis vinifera cv.Sultana)的分生组织培养中得到了强壮的组织和器官分化。不加6-苄基腺嘌呤,以萘乙酸替代吲哚乙酸同时添加0.1毫克/升赤霉酸诱导了愈合)组织)。然而,这种愈合组织被转移到含有吲哚乙酸和6-苄基腺嘌呤的新的培养基之后不能产生茎芽。为了使分生组织培养的茎芽诱导生根,采用一种内含1毫克/升吲哚乙酸,0.2毫克/升6-苄基腺嘌呤和10毫克/升盐酸半胱氨酸的培养基最为合适,同时为了小植株的不断产生,激素含量逐渐降低的培养基值得推荐。
On a MS-Nitsch medium containing 1 mg / l indoleacetic acid and 2 mg / l 6-benzyladenine (BA), shoots were harvested from meristem cultures of Vitis vinifera cv. Sultana In the strong organization and organ differentiation. Without 6-benzyladenine, naphthaleneacetic acid was substituted for indoleacetic acid while 0.1 mg / L GA3 was added to induce healing) tissue). However, this healing tissue failed to produce stem buds after being transferred to new media containing indoleacetic acid and 6-benzyladenine. In order to induce rooting of shoots cultured in meristems, it is most appropriate to use a medium containing 1 mg / l indoleacetic acid, 0.2 mg / l 6-benzyl adenine and 10 mg / l cysteine hydrochloride , At the same time in order to continue to produce small plants, hormones gradually reduce the medium is recommended.