Bach等(1969)描述了人类T淋巴细胞在体外可与绵羊红细胞(SRBC)形成E-玫瑰花(E-RFC),此后国内外许多作者在E-RFC试验的方法学上作了一些改进(1-7),但由于E-RFC试验影响因素较多、且由于T淋巴细胞和SRBC形成的玫瑰花不稳定,容易解离、因而各家的E-RFC正常值波动范围较大。有些作者报告,用δ-氨基乙基溴代硫脲(AET),神经氨酸酶及木瓜蛋白酶处理人外周血淋巴细胞或SRBC后,可促进两者间粘 Bach et al. (1969) described that human T lymphocytes can form E-rosettes (E-RFC) with sheep erythrocytes (SRBC) in vitro. Since then, many authors at home and abroad have made some improvements in the methodology of E-RFC tests ( 1-7), but due to the E-RFC test has many influencing factors, and because the rose formed by T-lymphocytes and SRBC is unstable, it is easy to dissociate, so the normal fluctuation range of E-RFC in each family is larger. Some authors have reported that treatment of human peripheral blood lymphocytes or SRBC with δ-aminoethyl bromothiourea (AET), neuraminidase, and papain can promote adhesion between the two.