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用农杆菌介导方法,将甜菜坏死黄脉病毒外壳蛋白(BNYVV CP)基因和丛根病外壳蛋白(BBSVCP)基因转化甜菜不同品系茎节外植体。以卡那霉素作为筛选标记基因,获得抗卡那霉素再生植株。再生植株经PCR扩增,获得目的基因大小的DNA片段,初步证明为转基因植株。
Using Agrobacterium-mediated method, the BNYVV CP and BBSVCP genes were transformed into the stem explants of different strains of sugar beet. Using kanamycin as a marker gene, kanamycin-resistant plants were obtained. The regenerated plants were amplified by PCR to obtain the DNA fragment of the target gene size, initially proved to be transgenic plants.