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目的:制备豚鼠变应性鼻炎(AR)动物模型,通过干预性处理研究内源性硫化氢(H2S)及其限速酶胱硫醚-γ-裂解酶(CSE)在AR中的变化及意义。方法:将24只豚鼠随机分为4组,分别为卵清蛋白致敏豚鼠变应性动物模型组(致敏组)、NaHS处理组及CSE抑制剂炔丙基甘氨(PPG)干预组(PPG组),同时以生理盐水处理豚鼠作为对照组,分别测定各组动物鼻腔灌洗液eotaxin、血浆H2S浓度,采用实时荧光定量RT-PCR法测定鼻黏膜中CSE的表达。结果:豚鼠致敏后eotaxin含量明显增加,而血浆H2S浓度及鼻黏膜CSE的表达明显低于对照组(P<0.05),而干预处理的NaHS组与致敏组相比,eotaxin相应减少(P<0.05),而H2S浓度及CSE的表达量增加(P<0.05),2组间差异有统计学意义,而经PGG干预处理后,eotaxin含量则增加,而H2S浓度及CSE的表达量降低,2组间差异有统计学意义(P<0.05),其中H2S含量与CSE的表达呈线相关。结论:内源性H2S参与AR的炎症过程,其含量主要受限速酶CSE的调控。
OBJECTIVE: To prepare the animal model of allergic rhinitis (AR) in guinea pigs and study the change and significance of endogenous hydrogen sulfide (H2S) and its rate-limiting enzyme cystathionine-γ-lyase (CSE) . Methods: Twenty-four guinea pigs were randomly divided into four groups: allergic animal model group (sensitized group), NaHS treatment group and CSE inhibitor propargylglycine (PPG) intervention group PPG group). At the same time, guinea pigs were treated with normal saline as the control group. The concentrations of eotaxin and plasma H2S in nasal lavage fluid of each group were measured. The expression of CSE in nasal mucosa was detected by real-time fluorescence quantitative RT-PCR. Results: The content of eotaxin in guinea pigs increased significantly, while the plasma concentration of H2S and the expression of CSE in nasal mucosa were significantly lower than those in control group (P <0.05), while the levels of eotaxin in NaHS group were lower than those in sensitized group (P <0.05), while the concentration of H2S and CSE increased (P <0.05). There was a significant difference between the two groups (P <0.05), while the content of eotaxin increased but the concentration of H2S and CSE decreased The difference between the two groups was statistically significant (P <0.05). The content of H2S was correlated with the expression of CSE. Conclusion: Endogenous H2S is involved in the inflammatory process of AR and its content is mainly regulated by the rate-limiting enzyme CSE.