蜕膜细胞是子宫内膜的间质细胞,在着床过程中由子宫的基质细胞分化而来并一直维持到妊娠终止。人蜕膜细胞离体培养容易生长,用离体培养的蜕膜细胞可以直接观察药物对人体子宫内膜的影响,是研究避孕药、具作用机理和宫内节育器引起子宫内膜出血机制的理想模型。用放射自显术观察离体培养的人蜕膜细胞,不仅可以避免整体实验时同位素对细胞的损害,而且可以在严格控制的条件下观察细胞的机能,有利于作单因素的分析。近年来,我们建立了离体培养人蜕膜细胞的放射自显影术,现简介如下。一、材料: 选从末次月经计算40～70天的人工流产的蜕膜组 Decidual cells are stromal cells of the endometrium that differentiate from the uterine stromal cells during implantation and persist until the end of pregnancy. Human decidual cells are easy to grow in vitro, the decidual cells cultured in vitro can directly observe the effects of drugs on human endometrium, which is a study of contraceptives with mechanism of action and mechanism of endometrial hemorrhage caused by IUD Ideal model Using autoradiography to observe the human decidual cells cultured in vitro can not only avoid the damage of isotope to the cells during the whole experiment, but also observe the function of the cells under the condition of strict control, which is good for univariate analysis. In recent years, we have established an autoradiograph to culture human decidual cells in vitro, and are summarized below. First, the material: Select from the last menstrual calculation of 40 to 70 days of abortion decidua group