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背景:氧应激在急性肝损伤的发生过程中具有重要作用,因此提高机体的抗氧化功能对防治急性肝损伤具有重要意义。目的:探讨维生素E(Vit E)和硒对CCl4大鼠急性肝损伤和抗氧化功能的影响。方法:48只健康雄性SD大鼠随机分为6组,每组8只。干预组第1周和第2周以及造模组第1周和第2周采用腹腔注射50% CCl4和橄榄油混合制剂的方式建立大鼠急性肝损伤模型,干预组在饲料中添加Vit E(250 mg/kg饲料)和硒(O.2 mg/kg饲料)进行营养干预,造模组喂饲标准饲料;对照组第1周和第2周腹腔注射生理盐水,喂饲标准饲料。第1周和第2周组大鼠分别于处理1周和2周后处死,分别检测各项肝损伤指标和抗氧化指标,并观察其含量变化。结果:对于急性肝损伤大鼠,补充适量的Vit E和硒能降低血清丙二醛(MDA)水平,干预组的血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)升高幅度明显较造模组小,肝组织羟脯氨酸含量亦相对低于造模组。造模组的肝组织和血清硒水平在第2周明显升高,而Vit E水平显著降低,并伴随着超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)活性的显著升高,而干预组的各项指标基本维持不变。结论:CCl4致大鼠急性肝损伤时,机体对抗氧化营养素的需求明显上升,补充Vit E和硒后,机体拮抗氧应激所致急性肝损伤的能力增强。
BACKGROUND: Oxygen stress plays an important role in the pathogenesis of acute liver injury. Therefore, improving the antioxidant function of the body is very important for the prevention and treatment of acute liver injury. Objective: To investigate the effects of vitamin E and selenium on acute liver injury and anti-oxidation in CCl4 rats. Methods: Forty-eight healthy male Sprague-Dawley rats were randomly divided into 6 groups with 8 rats in each group. The model of acute liver injury was established by intraperitoneal injection of 50% CCl4 and olive oil in the first and second week of the intervention group and the first and second week of the model group. The intervention group was given Vit E ( 250 mg / kg diet) and selenium (0.2 mg / kg diet). The rats in the model group were fed with the standard diet. The control group was given normal saline by intraperitoneal injection in the first week and the second week. The rats in the first week and the second week were sacrificed at 1 and 2 weeks after treatment, respectively, and the indicators of liver injury and anti-oxidation were detected, and the content of anti-oxidants were observed. Results: Serum levels of malondialdehyde (MDA) were decreased in rats with acute liver injury by supplementation of appropriate doses of Vit E and selenium, and serum ALT and AST increased Significantly smaller than the model group, liver hydroxyproline content is also relatively lower than the model group. The liver tissue and serum selenium levels in the model group increased significantly at the second week, while the Vit E level decreased significantly with the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) Significantly increased, while the intervention group’s indicators remained unchanged. Conclusion: CCl4-induced acute liver injury in rats, the body’s demand for antioxidant nutrients increased significantly, VitE and selenium supplementation, the body antagonistic oxygen stress-induced acute liver injury increased ability.