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Objective: To investigate the Reversal Effect of Irisquinone (ANKA) on Cisplatin-resistant Human Lung Adenocarcinoma Cell Line (A_(549)~(DDP)) and the change of MRP expression. Methods: MTT assay, flow cytometry, glutathione reductase recycling assay, RT-PCR were used. Results: None or low cytotoxic concentration of ANKA (10, 20, 30 (mol/L) could increase the sensitivity of A_(549)~(DDP) cells to CDDP by 8.2, 7.9 and 8.9-fold in a dose independent manner. After A_(549)~(DDP) cells was pretreated with 10 (mol/L ANKA for 12 h, CDDP cytotoxicity was increased 9.41-fold. The GSH content of the cells treated by ANKA is reduced significantly (P<0.001). The GST( protein expression was reduced by ANKA depended on its doses. ANKA also reduced expression of MRP protein, dependent on its dose and treating time (P<0.001). MRP mRNA expression was reduced only by 30 (mol/L ANKA (P<0.05). Conclusion: The reversal effect of ANKA on A_(549)~(DDP) cell was relative to intracellular glutathione system.
Objective: To investigate the Reversal Effect of Irisquinone (ANKA) on Cisplatin-resistant Human Lung Adenocarcinoma Cell Line (A_(549)~DDP) and the change of MRP expression. Methods: MTT assay, flow cytometry, glutathione reductase recycling assay , RT-PCR were used. Results: None or low cytotoxic concentration of ANKA (10, 20, 30 (mol/L) could increase the sensitivity of A_(549)~(DDP) cells to CDDP by 8.2, 7.9 and 8.9- After A_(549)~(DDP) cells was treated with 10 (mol/L ANKA for 12 h, CDDP cytotoxicity was increased 9.41-fold. The GSH content of the cells treated by ANKA is reduced significantly (P<0.001). The GST ( protein expression was reduced by ANKA depended on its doses. ANKA also reduced expression of MRP protein, dependent on its dose and treating time (P<0.001). MRP mRNA expression was only reduced by 30 ( Mol/L ANKA (P<0.05). Conclusion: The reversal effect of ANKA on A_(549)~(DDP) cell was relative to intracellular glutathione system.