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1990年4月下旬我们在准噶尔盆地古尔班通古特沙漠的南缘荒漠地区,从采集的亚洲璃眼蜱(Hyalomm asiaticum asiaticum)中分离到2株可引起乳鼠发病和在细胞培养中复制的病原。经血清学鉴定,包括交叉补体结合,间接免疫荧光和抗新疆出血热McAb(14B_7株)致敏血球的RPHA和RPHI试验证实为新疆出血热病毒。并采集了在荒漠牧场中放牧的羊群血清148份及放牧人员血清21份。用新疆出血热McAb RPHI法从羊血清中查到抗体阳性血清107份(72.3%)人群血清抗体阳性者2份(9.5%)。 上述结果首次证实了新疆北疆的准噶尔盆地南缘荒漠地带存在新疆出血热病毒的自然疫源地,该地区是北疆农牧业生产的主要地区和石油工业的新开发区,也是北疆主要的冬季牧场。
In the late April of 1990, we isolated 2 strains of Hyalomm asiaticum asiaticum in the southern margin of the Gurbantunggut Desert in the Junggar Basin, which can cause the onset of neonatal rat and replicate in the cell culture Of the pathogen. Serological identification, including cross-complement fixation, indirect immunofluorescence and anti-Xinjiang hemorrhagic fever McAb (14B_7 strains) sensitized blood cells RPHA and RPHI test confirmed as Xinjiang hemorrhagic fever virus. 148 grazing sheep and 21 grazing herds were grazed in desert pastures. In Xinjiang, hemorrhagic fever McAb RPHI method was used to detect two positive serum antibody (107.5%) in 107 (72.3%) serums from sheep serum. The above results for the first time confirmed the natural foci of Xinjiang hemorrhagic fever caused by Xinjiang hemorrhagic fever virus in the northern margin of the Junggar Basin in northern Xinjiang. The area is the major agricultural and livestock production area in northern Xinjiang and the newly developed oil industry. Winter ranch.