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实验用兔单特异抗艰难梭菌Ａ毒素ＩｇＧ包被酶标板，以羊抗艰难梭菌Ａ毒素ＩｇＧ标记辣根过氧化物酶作为第二抗体，采用双抗体夹心ＥＬＩＳＡ法检测艰难梭菌Ａ毒素，可检测出０．９４ｎｇ的精制Ａ毒素，对６１株菌的培养液及６５份健康人粪便标本检测发现此法具有较高的特异性。用平行线定量法对几份典型产毒培养物进行了定量测定，结果表明，在一定剂量范围内线性及平行性好，结果准确、可靠。可用于临床粪便标本中艰难梭菌Ａ毒素的筛查及定量检测。
In the experiment, rabbit monoclonal antibody against C. difficile A toxin IgG was used to coat ELISA plate, and goat anti-Clostridium A toxin IgG was used to label horseradish peroxidase as the second antibody. Double antibody sandwich ELISA was used to detect C. difficile A Toxin, 0.94 ng of refined A toxin was detected, and 61 strains of culture broth and 65 healthy human stool specimens were detected and found to have high specificity. Quantitative determination of several typical toxigenic cultures by parallel-line quantitation showed that the linearity and parallelism over a range of dosages was good and the results were accurate and reliable. Can be used for screening and quantitative detection of Clostridium difficile A toxin in clinical stool specimens.