为了探究植物生长调节剂多效唑(PP333)调控药用植物美洲商陆(Phytolacca americana)毛状根生长和次生代谢的可能性,设计实验并探讨PP333对美洲商陆毛状根生长及其商陆皂苷甲含量的影响。结果表明,与对照相比,PP333使毛状根根尖及侧根表面呈浅红色,侧根变得短而粗,且随着培养基内PP333浓度的升高,根表面颜色加深。培养基中添加0.5–5.0 mg·L–1 PP333能促进毛状根中商陆皂苷甲的产生,其中以1.0 mg·L–1 PP333的效果最好,其商陆皂苷甲含量达6.22 mg·g–1 DW,约为对照的1.94倍。PP333能提高毛状根苯丙氨酸裂解酶(PAL)的活性,并可能通过对PAL酶活性的调节来促进毛状根中商陆皂苷甲的产生。 In order to explore the possibility of PP333 regulating hairy root growth and secondary metabolism of medicinal plant Phytolacca americana, experiments were conducted to investigate the effects of PP333 on the growth of hairy roots of Piedmont Effect of content. The results showed that compared with the control, PP333 made the surface of root tips and lateral roots of hairy roots light red, the lateral roots became short and thick, and the root surface color deepened with the increase of PP333 concentration in the medium. The addition of 0.5-5.0 mg · L-1 PP333 to the medium promoted the production of esculentoside A in hairy roots, of which 1.0 mg · L -1 PP333 had the best effect and the content of celastrus glycosides was 6.22 mg · g-1 DW, about 1.94 times of the control. PP333 can increase the activity of hairy root phenylalanine lyase (PAL), and may promote the production of esculentoside A in hairy roots through the regulation of PAL enzyme activity.