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目的观察证实钙调蛋白Parvalbumin(PV)和Calbindin-28KD(CB)阳性神经元在大鼠脊髓不同节段不同区域内的形态及分布特征。方法成年雄性SD大鼠常规灌注固定,取第5～8颈髓、3～6胸髓和1～5腰髓节段;振动切片之后进行免疫组化PAP单标记;光镜观察阳性神经元的形态和分布特征、并进行计数和测量;用Excel软件对阳性神经元数量、胞体大小、突起数量进行统计处理。结果PV阳性神经元主要存在于后角的Ⅱ层和胸核、中间带外侧、中间内侧核、前角的Ⅷ层;CB阳性神经元主要存在于后角Ⅰ～Ⅱ层、中间带,前角的Ⅷ～Ⅸ层之间。后角细胞较中间带及前角细胞数量多,胞体小,突起少。在不同区域,两种阳性神经元的细胞数量,胞体大小,突起数目都存在统计学差异(P﹤0.05);而在不同节段同一区域的比较中,只有腰前角PV阳性神经元的胞体比较大(P﹤0.05)。结论PV阳性神经元在胸核及中间内侧核的聚集以及阳性纤维在薄楔束和后角Ⅱ层胶状质内的密集分布提示其与机体痛觉传入﹑本体感觉及内脏感觉的联系;CB阳性神经元在前角Ⅷ～Ⅸ层之间的特征性分布说明其与闰绍细胞功能有关;两种钙调蛋白在脊髓不同节段之间的形态及分布基本无差别提示它们的功能并没有特异地对应于躯体或内脏。
Objective To investigate the morphological and distribution characteristics of Parvalbumin (PV) and Calbindin-28KD (CB) positive neurons in different regions of rat spinal cord. Methods Adult male Sprague-Dawley rats were routinely perfused and fixed. The fifth to eighth cervical spinal cord, 3 to 6 thoracic spinal cord and 1 to 5 lumbar spinal cord segments were harvested. Vibrational sections were harvested for immunohistochemical staining with PAP. The positive neurons Morphology and distribution characteristics, and counting and measurement; the number of positive neurons, cell body size, the number of protrusions were statistically calculated using Excel software. Results PV-positive neurons were mainly found in the posterior horn of the second layer and the chest nucleus, the medial zone, medial medial nucleus, anterior horn Ⅷ layer; CB positive neurons mainly in the posterior horn Ⅰ ~ Ⅱ layer, the middle zone, Ⅷ ~ Ⅸ layer between. Posterior horn cells than the median and anterior horn cell number, cell body is small, less protrusions. In different regions, there were significant differences in the number of cells, cell body size and the number of neurites in both positive neurons (P <0.05), while in the same region of different segments, only the cell bodies of PV positive neurons Greater (P <0.05). Conclusions The accumulation of PV-positive neurons in the nucleus pulposus and the medial medial nucleus, and the dense distribution of positive fibers in the laminar layer of the amygdala and posterior horn suggest the relationship between PV-induced neuronal pain and proprioceptive sensation and visceral sensation. CB The characteristic distribution of positive neurons in the Ⅷ ~ Ⅸ layers indicates that they are related to the function of intercalated cells. The two types of calmodulin are basically indistinct in the morphology and distribution of different segments of the spinal cord, suggesting that their function is not Specific to the body or internal organs.