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目的观察球囊损伤后大鼠腹主动脉富含半胱氨酸蛋白61、ERK1/2的表达及氯沙坦对富含半胱氨酸蛋白61、ERK1/2表达的影响,探讨氯沙坦抑制动脉增殖的机制。方法72只雄性Wistar大鼠分为氯沙坦组、球囊损伤组和假手术组。对球囊损伤后不同时间段的大鼠腹主动脉标本采用免疫组织化学、免疫蛋白印记分析检测富含半胱氨酸蛋白61、ERK1/2的表达。结果7 d和14 d球囊损伤组富含半胱氨酸蛋白61、ERK1/2的表达均明显增强,与假手术组比较有显著差异(P<0.01),氯沙坦组与球囊损伤组比较均明显降低。28 d时,3组两两比较没有显著差异(P>0.05)。结论氯沙坦抑制球囊损伤后富含半胱氨酸蛋白61、ERK1/2的表达,提示氯沙坦抑制球囊损伤后内膜增生可能是通过抑制富含半胱氨酸蛋白61发挥作用的,这种抑制作用可能部分是通过ERK1/2信号转导途径介导的。
Objective To investigate the expression of cysteine protein 61 and ERK1 / 2 in abdominal aorta of rats after balloon injury and the effect of losartan on the expression of cysteine-rich protein 61 and ERK1 / 2 in losartan Inhibition of arterial proliferation mechanism. Methods Seventy-two male Wistar rats were divided into losartan group, balloon injury group and sham operation group. The specimens of abdominal aorta of rats at different time points after balloon injury were used to detect the expression of cysteine-rich protein 61 and ERK1 / 2 by immunohistochemistry and immunoblotting. Results The levels of cysteine protein 61 and ERK1 / 2 were significantly increased in balloon injury group on day 7 and day 14 compared with sham-operated group (P <0.01). Losartan group and balloon injury Group comparison were significantly lower. 28 d, there was no significant difference between the two groups (P> 0.05). Conclusions Losartan inhibits the expression of cysteine protein 61 and ERK1 / 2 after balloon injury, suggesting that losartan may inhibit neointimal hyperplasia after balloon injury by inhibiting cysteine rich protein 61 The inhibitory effect may be partly mediated by ERK1 / 2 signal transduction pathway.