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目的 探求白细胞介素 6 (IL 6 )与癫痫发病之间的关系。方法 将 6 1只Wistar大鼠分成 3组 ,实验组侧脑室注入马桑内酯 (CL) ,2 μl(0 0 5mg/kg体重 ) ,生理盐水对照组注入等量的生理盐水 ,空白对照组不做任何处理。实验中对大鼠的行为及脑电图 (EEG)进行观察和记录 ;同时运用免疫组织化学方法 ,对大鼠海马IL 6的免疫反应性进行分析。结果 实验组大鼠侧脑室注射CL过程中即诱发癫痫 ,且测到痫性脑电图 ,生理盐水对照组、空白对照组无癫痫症状及癫痫样脑电图出现 ;实验组大鼠海马CA1、CA2 区IL 6免疫反应性较生理盐水对照组及空白对照组显著增强 (P <0 0 1) ,表现为海马CA1、CA2 区阳性反应细胞数目增多 ,着色明显加深 ;生理盐水组和空白对照组之间差异无显著性意义 (P >0 0 5 )。结论 癫痫的发病过程中海马神经元内的IL 6含量有显著的变化 ,二者之间可能存在着密切的联系。
Objective To explore the relationship between interleukin 6 (IL 6) and epilepsy. Methods Sixty-one Wistar rats were divided into three groups. The experimental group was injected intracerebroventricular injection with 2 mg (2.5 mg / kg body weight) of coronal lactone, the normal saline control group was injected with normal saline, and the blank control group Do not do anything. In the experiment, the behavior and electroencephalogram (EEG) of rats were observed and recorded. At the same time, immunoreactivity of IL 6 in rat hippocampus was analyzed by immunohistochemical method. Results In the experimental group, epilepsy was induced in the process of intracerebroventricular injection of CL, epileptiform electroencephalogram (EEG), saline control group and blank control group were found without epilepsy symptoms and epileptiform EEG. In the experimental group, hippocampal CA1, Compared with saline control group and blank control group, the immunoreactivity of IL-6 in CA2 area was significantly increased (P <0.01), and the number of positive cells in CA1 and CA2 area of hippocampus increased and the coloration was significantly deepened. Compared with the control group There was no significant difference between the two groups (P> 0.05). Conclusion There is a significant change of IL 6 content in hippocampal neurons during the onset of epilepsy, and there may be a close relationship between the two.