论文部分内容阅读
以超速离心法制备大鼠肝刺激因子（ＨＳＳ）。采用无血清培养技术培养大鼠肝细胞并观察ＨＳＳ作用肝细胞后不同时刻肝细胞ＥＧＦ受体ｍＲＮＡ表达的变化。结果显示，ＨＳＳ可刺激ＥＧＦ受体ｍＲＮＡ的表达。给药后２～６ｈ，ｍＲＮＡ表达达高峰。ＨＳＳ与ＥＧＦ合用可明显刺激受体ｍＲＮＡ表达。Ｎｏｒｔｈｅｒｎ杂交分析表明，ｍＲＮＡ表达明显增高系由于５．６和１０．０ｋｂ两基因片段所致，提示ＨＳＳ可在基因水平调节ＥＧＦ受体活性。本工作进一步证明ＨＳＳ对ＥＧＦ受体的调节可能为其促肝再生的重要机制之一。
Rat hepatic stimulating factor (HSS) was prepared by ultracentrifugation. Serum-free culture of rat hepatocytes was used to observe the changes of EGFR mRNA expression in hepatocytes at different time points after HSS treatment. The results show that HSS can stimulate the expression of EGF receptor mRNA. After 2 ~ 6h after administration, mRNA expression peaked. HSS combined with EGF can obviously stimulate the receptor mRNA expression. Northern blot analysis showed that mRNA expression was significantly increased due to the 5.6 and 10.0 kb two gene fragments, suggesting that HSS can regulate EGF receptor activity at the gene level. This work further demonstrated that HSS EGF receptor regulation may be one of the important mechanisms of promoting liver regeneration.