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目的:观察姜黄素对大鼠局灶脑缺血/再灌注后神经细胞凋亡的作用及其机制。方法:采用SD大鼠线栓模型,动物随机分为3组:假手术组(sham组)、缺血/再灌注组(vehicle组)、姜黄素100 mg.kg-1组(Cur 100组),TUNEL法检测皮层神经细胞凋亡;Western-blot检测凋亡诱导因子(AIF)和细胞色素C(Cytc)在缺血皮层的表达;同时检测膜电位的变化。结果:I/R组凋亡细胞多于sham组及Cur 100组,Cur 100能够显著改善I/R所引起的线粒体膜电位下降;降低AIF,Cytc的表达。结论:姜黄素能够显著减少缺血性神经细胞凋亡的发生,可能与保护线粒体功能,减少AIF和Cytc的释放有关。
Objective: To observe the effect of curcumin on neuronal apoptosis after focal cerebral ischemia / reperfusion in rats and its mechanism. Methods: The rats were randomly divided into sham operation group (sham group), ischemia / reperfusion group (vehicle group), curcumin 100 mg.kg-1 group (Cur 100 group) The apoptosis of cortical neurons was detected by TUNEL method. The expressions of apoptosis-inducing factor (AIF) and cytochrome C (Cytc) in ischemic cortex were detected by Western-blot. The changes of membrane potential were also detected. Results: The number of apoptotic cells in I / R group was higher than that in sham group and Cur 100 group. Cur 100 could significantly decrease the mitochondrial membrane potential induced by I / R and decrease the expression of AIF and Cytc. Conclusion: Curcumin can significantly reduce the occurrence of ischemic neuronal apoptosis, which may be related to the protection of mitochondrial function and the release of AIF and Cytc.