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目的:研究在氟中毒引起氟斑牙时,氟对大鼠切牙细胞增殖和DNA损伤的影响。方法:给雄性SD大鼠饮用含10,50,100mg/LNaF的高氟水60d和90d,制备氟斑牙模型,用流式细胞术检测大鼠切牙细胞增殖周期的变化,用单细胞凝胶电泳检测DNA损伤。结果:雄性SD大鼠饮用含50,100mg/LNaF的高氟水60d和90d,血清氟含量较对照组显著增高,P<0.05,具有较好的剂量-效应关系,相关系数分别为:0.9957(P<0.01)和0.9880(P<0.05)。在50,100mg/LNaF剂量条件下,大鼠切牙呈现典型的氟斑牙改变。低氟组(10mg/LNaF)大鼠60d和90d,均没有明显氟斑牙形成。在10,50和100mg/LNaF的剂量条件下染毒60d和90d,大鼠切牙细胞出现明显的DNA损伤,Olive尾距较对照组显著增高(P<0.05),而且在相同剂量条件下,染毒90d时的Olive尾距值比染毒60d时的Olive尾距值大(P<0.05)。在10,50和100mg/LNaF的剂量条件下染毒60d,大鼠切牙细胞增殖周期变化主要表现在,低氟组G2/M细胞显著减少,中氟组和高氟组的G2/M细胞明显增多。染毒90d,大鼠切牙细胞增殖周期却没有明显改变。结论:在氟中毒引起氟斑牙的过程中,大鼠切牙细胞增殖周期改变和DNA损伤发挥作用,但详尽机制仍需深入探讨。
AIM: To investigate the effect of fluoride on proliferation and DNA damage of rat incisor teeth during fluorosis induced dental fluorosis. Methods: Male SD rats were fed with high-fluoride water containing 10, 50 and 100 mg / L NaF for 60 days and 90 days, respectively. The dental fluorosis model was established by flow cytometry. The single cell gel Electrophoresis detects DNA damage. RESULTS: After 60 and 90 days of drinking high-fluoride water containing 50 and 100 mg / L NaF, the serum fluorine content of male SD rats was significantly higher than that of the control group (P <0.05), and the correlation coefficient was 0.9957 <0.01) and 0.9880 (P <0.05). At 50 and 100 mg / LNaF, rat incisors showed typical changes of dental fluorosis. Low fluoride group (10mg / LNaF) rats 60d and 90d, no obvious dental fluorosis formation. At the doses of 10, 50 and 100mg / LNaF for 60d and 90d, there were obvious DNA damage in the rat incisor cells, the tail of Olive was significantly higher than that of the control group (P <0.05), and under the same dosage, Olive tail value at 90 d after exposure was higher than that at 60 d after exposure (P <0.05). At 60, 50 and 100 mg / L NaF, the cell cycle changes were mainly observed in G2 / M cells in low fluoride group and G2 / M cells in middle fluoride group and high fluoride group Significantly increased. After exposure to 90 days, the rat periodontal cell proliferation cycle did not change significantly. Conclusion: In the process of fluorosis induced dental fluorosis, the changes of rat periodontal cell proliferation cycle and DNA damage play a role, but the detailed mechanism still needs further study.