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用成年猫作脊髓半横贯切割损伤模型,即从SZ节段在右侧半切取约3mm长的脊髓。术后28天经左心室主动脉插管冲洗,然后经多聚甲醛戊二醛灌注固定。取L6、SZ、S4节段背侧Ⅱ板层电镜观察。术后SZ节段Ⅱ板层的简单终末(小、单突触)、粗细有髓纤维、Ⅰ型复合终末(电子密度较高,会大小不一分布均匀的突触小泡,线粒体量少)和Ⅱ型复合终未(电子密度较低,含大小相近但密度不一的突触小泡,粒线体数量多)均增多。左侧半从上至下(L6、SZ、S4)粗钢有髓纤维,简单终末数、Ⅰ型复会终未均逐步增多(Ⅱ型逐步减少)。右侧半,与术前SZ节相比,L6节的简单终未、细有髓纤维和髓鞘增厚且玻璃样变的粗有髓纤维大量存,同时有对称性突触的小偏平终末出现;S4节则无髓纤维和Ⅰ型复合终天增多,简单终末数减少,Ⅱ型复合终未很少。术后与术前相比,神经元胞质内溶酶体、线粒体、粗面内质网明显增多。线粒体脊变明显;神经胶质细胞胞突内的胶质丝明显增多。上述结果表明:满脊髓半横贯损伤后结构上是增生与演变同时存在,但增生在损伤侧以上和以下的节段及两侧之间有较大差别,即损伤平面以上及对侧半可能是来自后根的无髓纤维和下行的投射纤维的出芽同时发生,而损伤平面以下可能只有来自后根的无阻纤维出芽。
A semi-traumatic spinal cord transection lesion model was performed in adult cats, ie about 3 mm long of the spinal cord was cut in the right half from the SZ segment. 28 days after the left ventricular aortic cannulation, and then by paraformaldehyde glutaraldehyde perfusion fixed. Take L6, SZ, S4 segment dorsal Ⅱ plate electron microscopy. Simple terminal (small, monosynaptic), thick myelinated fibers, type Ⅰ complexed terminals (synaptic vesicles with high electron density, uniform distribution of size, amount of mitochondria Less) and type Ⅱ complex (electron density is lower, with similar size but different density of synaptic vesicles, the number of mitochondria) increased. Left half from top to bottom (L6, SZ, S4) crude steel medullary fibers, simple terminal number, type I complex will not gradually increase the average (type Ⅱ gradually reduced). Right and a half, compared with the preoperative SZ section, L6 section of the simple end, fine myelinated and myelinated thickened and glassy coarse myelinated fibers in large deposits, at the same time the symmetrical synapse of small flat end S4 segment then the myelinated fibers and type Ⅰ complex increased all the time, the number of simple terminal decreased, type Ⅱ complex is not very few. After surgery compared with preoperative, neuronal cytoplasm lysosomes, mitochondria, rough endoplasmic reticulum increased significantly. Mitochondrial ridges obvious; glial cell glial glial increased significantly. The above results show that: after the full spinal cord traversal injury is the proliferation and evolution of the structural existence, but hyperplasia in the injured side above and below the segments and between the two sides are quite different, that is, above the injury plane and the contralateral half may be Unpulmonary fibers from the posterior root and descending projective fibers sprout simultaneously, while only the unimpeded fibers from the posterior root below the injury plane may bud out.