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本文报告妊娠小鼠分别于妊娠后第七天和十六天腹腔注射内蒙古风化煤腐植酸钠,于妊娠第十七天处死,取材制片,观察成鼠骨髓及胎鼠血液、胎肝细胞的微核出现率。实验分四组,第一组剂量为40mg/kg,第二组为80mg/kg,第三组为阳性对照组,腹腔注射环磷酰胺40mg/kg,第四组为阴性对照组,腹腔注射等量蒸馏水。实验结果:40mg/kg 组和80mg/kg组,成鼠骨髓嗜多染红细胞的微核检出率与阴性组比较,无明显差别(p〉0.20);胎鼠血液和胎肝嗜多染红细胞的微核检出率与阳性对照组比较也无明显差男别(p〉0.05)。结果提示:40mg/kg—80mg/kg 内蒙古风亿煤腐植酸钠对成鼠及其胎鼠无明显细胞毒性作用。
This article reports that pregnant mice were intraperitoneally injected with humic acid in Inner Mongolia weathered coal on the seventh and the 16th day after gestation. They were sacrificed on the 17th day of gestation. Samples were taken to observe the blood and fetal liver cells of adult rat bone marrow and fetal rat. Micronucleus frequency. The experiment was divided into four groups, the first group of dose was 40mg/kg, the second group was 80mg/kg, the third group was a positive control group, intraperitoneal injection of cyclophosphamide 40mg/kg, the fourth group was a negative control group, intraperitoneal injection, etc. Amount of distilled water. Experimental results: In the 40 mg/kg group and 80 mg/kg group, the detection rate of micronucleus in adult rat bone marrow polychromatic erythrocytes was not significantly different from that in the negative group (p>0.20); fetal rat blood and fetal liver polychromatic erythrocytes The micronucleus detection rate was not significantly different from the positive control group (p>0.05). The results suggest that 40 mg/kg-80 mg/kg of humic acid in Inner Mongolia Fengyi Coal has no obvious cytotoxic effect on adult and fetal rats.