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流式细胞术是近年来发展的一项新技术,应用该项技术的重要环节是制备合格的单分散细胞悬液。本文对食管癌组织、脱落细胞和血液标本的悬液制备方法作了初步探讨,结果表明,胶原酶对实体肿瘤组织单细胞悬液制备是可行的。脱落细胞和血液样品容易得到合格的细胞悬液。并对影响流式细胞分析的一些因素作了简单的讨论。
Flow cytometry is a new technology that has been developed in recent years. An important part of applying this technology is the preparation of a qualified monodisperse cell suspension. In this paper, the preparation method of the suspension of esophageal cancer tissue, exfoliated cells and blood samples was discussed. The results showed that collagenase is feasible for the preparation of single cell suspension of solid tumor tissue. Exfoliated cells and blood samples are easily given a qualified cell suspension. Some of the factors that influence the flow cytometry analysis are briefly discussed.