论文部分内容阅读
背景:儿茶素是一种抗氧化能力很强的天然植物抗氧化剂,因其稳定性较差且生物利用率较低,需对剂型加以改进以提高其效能。目的:研究儿茶素微胶囊对肾病综合征大鼠肾功能的影响。设计:随机对照研究。单位:湖南农业大学生化与发酵工程实验室和中南大学湘雅二医院小儿肾病研究室。材料:实验在上述两单位的实验室完成。选取6周龄体质量相近(180～220g)的SD雌性大鼠120只,随机分成对照组、肾病组、激素治疗组、VitE治疗组、儿茶素治疗组、儿茶素微胶囊治疗组,每组20只。方法:实验第1天对照组尾静脉1次注射0.1mL生理盐水,其他各组尾静脉1次注射阿霉素(5mg/kg)。激素治疗组自实验第2周末皮下注射地塞米松犤1.8mg/(kg·d)犦至第6周末实验结束。VitE治疗组自实验第2周末行最大剂量维生素E灌胃犤50mg/(kg·d)犦至第6周末实验结束。儿茶素治疗组自实验第2周末行儿茶素灌胃犤50mg/(kg·d)犦至第6周末实验结束。儿茶素微胶囊治疗组自实验第2周末行儿茶素微胶囊灌胃犤50mg/(kg·d)犦至第6周末实验结束。应用考马斯亮蓝法测定不同时期24h尿蛋白排泄量,采用半定量评分法分别对肾小球、肾小管间质的病理改变进行计分评价。主要观察指标:大鼠24h尿蛋白排泄量和肾脏病理积分。结果:实验末,24h尿蛋白的排泄量依次为:肾病组>Vi
BACKGROUND: Catechin is a natural plant antioxidant with strong antioxidant capacity. Due to its poor stability and low bioavailability, catechin needs to be improved to improve its efficacy. Objective: To study the effect of catechin microcapsule on renal function in nephrotic syndrome rats. Design: Randomized controlled study. Unit: Biochemical and Fermentation Engineering Laboratory, Hunan Agricultural University and Xiangya Second Hospital of Central South University Pediatric Nephropathy Laboratory. Materials: The experiment is done in the above two labs. A total of 120 SD female rats aged 6 weeks with similar body weight (180 ~ 220g) were randomly divided into control group, nephropathy group, hormone therapy group, VitE treatment group, catechin treatment group, catechin microcapsule treatment group, Each group of 20. Methods: On the first day of experiment, the tail vein of the control group was injected with 0.1 mL of normal saline once, and the other groups were injected with doxorubicin (5 mg / kg) once a day. Hormone treatment group from the second week of experimental subcutaneous injection of dexamethasone 犤 1.8mg / (kg · d) 犦 until the end of the sixth week of the experiment. VitE treatment group from the end of the second experiment the maximum dose of vitamin E intragastric administration 犤 50mg / (kg · d) 犦 until the end of the sixth week of the experiment. Catechin treatment group from the second weekend of the experiment line catechin gavage 犤 50mg / (kg · d) 犦 until the end of the sixth week of the experiment. Catechin microcapsule treatment group from the second weekend of the experimental line of catechin microcapsules intragastric administration 犤 50mg / (kg · d) 犦 until the end of the sixth week of the experiment. Urine protein excretion was measured by Coomassie brilliant blue method at different time points, and the pathological changes of glomerular and tubulointerstitial were scored by semi-quantitative scoring method. MAIN OUTCOME MEASURES: 24h urinary protein excretion and renal pathological score. Results: At the end of experiment, urinary protein excretion in 24h was: nephrosis> Vi